Devise a scheme to prepare 10^-6 dilution on a plate using the least number of sterile water dilution tubes.

Answers

Answer 1

Answer:

The serial dilution can be done by half- logarithmic dilution method.

Explanation:

The following step explains the way to obtain [tex]\frac{1}{10^6}[/tex] dilution. The volumes taken are arbitrary.Take 1 micro-litre (ul)  of the concentrated solution in a dilution tube. Add water to it till the total volume becomes 10 ml. Here the solution formed is [tex]\frac{1}{10^4}[/tex] diluted.Take 100 micro-litre(ul) of [tex]\frac{1}{10^4}[/tex] diluted solution to another tube and add 9900 micro-litre(ul) of water to it. Here the solution formed is [tex]\frac{1}{10^6}[/tex] diluted.

Answer 2
Final answer:

To prepare a 10^-6 dilution with the least number of sterile water tubes, perform a series of serial dilutions, starting with a 1:10 dilution and repeatedly diluting the resulting solution by the same ratio until the desired 10^-6 dilution is reached.

Explanation:

1. Make a 1:10 dilution by taking 1 unit of A (such as 1 ml) and adding it to 9 units of sterile water. This will give you a 10^-1 dilution (B).

2. Repeat this process with B to get the 10^-2 dilution (C).

3. Continue this process until you reach the desired 10^-6 dilution.

This method ensures that you are using the least number of tubes by diluting sequentially rather than trying to make the 10^-6 dilution directly, which would require a tremendous amount of sterile water and be significantly less accurate.

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Related Questions

Pollution from a single, identifiable source is called
source pollution.

Answers

Pollution from a single, identifiable source is called point source pollution.

Explanation:

The things that we use for the production of anything releases some wastes products that act as a pollutant to  the environment. these are classified into two main category such as Point source and non point source pollution. In non point source pollution the cause for the pollution is from many places and it is very tedious to find the source of the pollution.

When a pollution occurs from a single and also an identifiable place or source then it is called as Point source pollution. Some of the examples of point source pollution includes, Factories, power plants, Municipal wastewater treatment plants,etc.

Final answer:

Pollution from a single, identifiable source is referred to as point-source pollution, which is easier to manage than nonpoint-source pollution due to its identifiable nature.

Explanation:

Pollution from a single, identifiable source is called point-source pollution. This type of contamination of the environment occurs when pollutants are released into the environment from a discernible, confined place. Examples of such single-source pollution include discharges from a pipe at a factory, overflows from combined sewer systems, leaks from storage tanks, or chemical spills. Conversely, nonpoint-source pollution is diffuse and originates from multiple, distributed sources.

In comparison, point-source pollution is generally easier to identify, monitor, and regulate, because the source is known. Nonpoint-source pollution, on the other hand, is more challenging to control, as it comes from a multitude of sources, like runoff from urban areas or agricultural fields that carry contaminants into bodies of water. It's important to understand the difference between these two types of pollution to effectively tackle environmental contamination.

Active transport requires energy because it usually involves the movement of
A.
substances from an area of low concentration to an area of high concentration.
B.
substances from an area of high concentration to an area of low concentration.
C.
substances in the gas phase, such as oxygen and carbon dioxide.
D.
substances that can easily pass through the cell membrane, such as water.

Answers

A. Substances from an area of low concentration to an area of high concentration.

Active transport requires energy because it usually involves the movement of substances from an area of low concentration to an area of high concentration.

What is active transport?

Active transport is the movement of a substance into or out of the cell. The movement is against the concentration gradient, and the movement is from lower concentration to higher concentration.

Example is diffusion.

Thus, the correct option is A.

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What are pharyngeal pouches, and how are
they evidence of evolution?

Answers

Answer:

Their similarity

Explanation:

Pharyngeal pouches are structures in vertebrates that develop in different parts of the body mainly in the face or neck. The similarity in their position and structural indicates a similarity . These structures provide evidence of evolution that the organisms evolved from the same ancestry.

A pharyngeal pouch (also known as Zenker's diverticulum) is a tiny bulge or pocket in the pharynx, similar to a hernia.

Pharyngeal pouches are transformed into different organs during evolution. This shows the relationship between different organisms using the pharyngeal pouch for different use.

Pharyngeal pouch

In embryological development, pharyngeal pouches are endodermal out-pockets that arise between the pharyngeal arches.

The pouches give rise to tissues that comprise the middle ear cavity and Eustachian tube, as well as the palatine tonsils, thymus, parathyroid glands, and thyroid parafollicular cells.

Thus, this is how the pharyngeal pouches are the evidence of evolution.

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Sierra decides to write down everything she likes to eat. Then, she draws branches under each animal on the list and writes everything they like to eat, and so on until she is down to all plants at the bottom of the page. What did Sierra make a diagram of?

Answers

Sounds like she made a food chain!

6. Adaptations that result from natural selection are expected to increase the fitness of an organism.
In terms of natural selection, which of the following best describes fitness?
a. Being able to escape from predators
b. Being among the strongest organisms in a population
c. Being able to survive, find a mate, and produce offspring
d. Being able to survive long enough to reach the adult stage

Answers

Answer:

C: Being able to survive, find a mate, and produce offspring

Explanation:

The population is an important factor to the increased fitness of the organism, being able to reproduce would lead to the population of that organism to be more healthy.

Adaptation is the process by which a species adapts to its surrounding environment during evolution. Being able to survive, find a mate, and produce offspring, best describe the fitness of the organism.

Natural Selection:

It is the process by which the most appropriate organism survives and increases its population.

In a population, organisms vary in some traits, these traits may increase the chances of survival of individual over others.The variation can also decrease the chances of survival.For example, A red color butterfly is more susceptible to being eaten by predators while for a green color butterfly, there are more chances of survival.

Therefore, Being able to survive, find a mate, and produce offspring, best describe the fitness of the organism.

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Work a punnett square for a father who is type A heterozygous and the mother is type B heterozygous.

What is the phenotypic ratio?

Answers

Answer:

Work a punnett square for a father who is type A heterozygous and the mother is type B heterozygous.

Ab X Ba = AB, Aa, Bb, and ab

Explanation:

Father type A  has 50% while type B which is the mother has 25% and while 25% co-recessive trait.

Which equation best represents the process of photosynthesis

Answers

6CO2+12H2O+light->C6H12O6+6O2+6H2O

Zooplankton get their energy from
phytoplankton
grasses
photosynthesis
small fish

Answers

Answer:

Zooplankton -tiny aquatic habitat organisms- gets their energy from phytoplankton consumption.

Explanation:

Zooplankton, or animal plankton, is characterized by being heterophosphoric organisms, obtaining its energy by consuming organic matter.

The main food source of zooplankton is phytoplankton, although they can also feed on organic waste, from the same zooplankton or even bacteria.

Zooplankton is composed of a very small fauna that includes single-cell organisms -such as protozoa and protists- fingerlings,  larval forms of mollusks, crustaceans and aquatic arthropods.

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When astronauts sleep in space, they sleep in small cabins or in sleeping bags that are attached to the wall of the station.

Which condition requires them to attach their sleeping bags to the wall?

Answers

Answer:

Low gravity

Explanation: i would assume? :)

Answer:the answer is FREE FALL

Explanation:

Just answered it


True or false: even when your research does not support your hypothesis, the hypothesis has value.

Answers

Answer:

True

Explanation:

Why are estuaries, such as the Pamlico Estuary, so important?

Answers

Answer:

An estuary can be described as an area of brackish water where different rivers or streams meet the open sea. Hence, an estuary forms a transition zone between an river environment and a marine environment.

Estuaries hold a lot of importance and are referred to as the nurseries of the sea. The estuary are home to many unique kinds of organisms. Certain kinds of fishes and shellfishes which humans consume cone from this area of the sea. There are high levels of nutrients moving in the estuary zone of the sea. Due to its shallow water, Pamlico estuary provides opportunities for fishing, crabbing and water- sports activities.

Estuaries like the Pamlico Estuary are crucial due to their commercial and biological significance.

Estuaries, such as the Pamlico Estuary, play a significant role both commercially and biologically.

They are home to many of the world's metropolitan areas, serving as ports for industrial activity and supporting large human populations.Biologically, estuaries are incredibly important because they act as nursery grounds for many species of fish, birds, and invertebrates. The mixing of fresh and salt water in estuaries creates a brackish environment that is rich in nutrients, promoting biodiversity and sustaining various life forms.Salinity in estuaries varies with the influx of seawater and outflow of freshwater, which influences the types of organisms and their adaptations. Many species in estuaries have developed specialized adaptations to cope with these varying salinity levels.Furthermore, estuaries contribute to the economy by providing areas for industrial activities and ports. These areas also offer recreational opportunities and help in filtering pollutants from rivers before they reach the ocean, thus maintaining environmental health.

All of the following statements were part of the original cell theory proposed by Schleiden and Schwann, and many others including Remak and Virchow, with the EXCEPTION of

A) all cells come from other cells.
B) the cell is the most basic unit of life.
C) all living things are composed of one or more cells.
D) viruses are not living since they are smaller than a cell.

Answers

Answer:

viruses are not living since they are smaller than a cell.

Explanation:

Have a good day :)

The viruses are not living as they are smaller than a cell. Thus the option D is correct.

What is the cell theory of schledien and Schwann?

The botanist and zoologists were studying tissues and proposed a theory that states that living things are made up of one or more cells and they are a basic unit of life. The new cells grow from the existing ones.

They gave the theory in 1838 on the basis of a comparison of plants and animal cells. The virus don't have cells that are larger enough and hence they are not living.

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How have modern advancements allowed this modern-day scientist to do research in a way that Darwin couldn't?

Answers

Answer: unlike modern-day evolutionary geneticists, Darwin did not have the knowledge of modern-day genetics or the ability to sequence the genome. So, Darwin couldn’t have determined that the trait of flightless wings was likely caused by a genetic mutation.

Explanation:

Answer:

Unlike modern-day evolutionary geneticists, Darwin did not have the knowledge of modern-day genetics or the ability to sequence the genome. So, Darwin couldn’t have determined that the trait of flightless wings was likely caused by a genetic mutation.

Explanation:

Answer from PLATO

Temperate grasslands and
are two biomes that are dominated by grass species, share similar wildlife, and have
few
. However, while temperate grasslands have
the other grassland biome
has
Reset
Next

Answers

Answer:

Temperate grasslands and tropical savannas are two biomes that are dominated by grass species, share similar wildlife, and have few trees. However, while temperate grasslands have hot summers and cold winters, the other grassland biome has both rainy and dry seasons

these are the correct answes

Explanation:

Answer:

First Blank: tropical savannas

Second Blank: trees

Third Blank: hot summers and cold winters

Fourth Blank: both rainy and dry seasons

[tex]\hrulefill[/tex]

First Blank:Another biome of grass lands is called the tropical savannas and like the temperate grassland, the tropical savannas are also majorly composed of different species of grasses and holds similar species.

Second Blank:Temperate grasslands and tropical savannas also have few trees and this is one of the important features that define grassland biomes.

Third Blank:Temperature grasslands are characterized by regional climates with variations which are: hot summers and cold winters.

Fourth Blank:Tropical savannas, have areas with more rainfall and those with less rainfall and this determines the nature of the vegetation and animal life in the region.

[tex]\hrulefill[/tex]

Choose two of the early cell formation hypotheses and discuss their differences:

Answers

The two different hypotheses of the origin of early cell structure are the iron-sulfide bubbles hypothesis and the lipid membrane hypothesis

Explanation:

The iron-sulfide bubbles hypothesis by William Martin and Micheal Russel originated from small-sized compartments formed with iron sulfide bubbles which were lying on the ocean floor. These compartments had organic matter trapped inside them and which developed further and started to be bounded by their own cell membrane.  

The lipid membrane hypothesis by Harold Morowitz proved that liposomes with the double lipid membrane formed organic material and could be separated from the environment. This theory stated that the cells were formed by the surrounding organic molecules .

The "heterotrophic origin" posits cells relied on external organic molecules, while the "autotrophic origin" suggests cells synthesized their own from inorganic sources.

Two early hypotheses regarding the formation of cells are the "heterotrophic origin" and the "autotrophic origin."

1. **Heterotrophic Origin**: This hypothesis suggests that the first cells were heterotrophic, meaning they relied on pre-existing organic molecules for energy and carbon sources.

According to this theory, these primitive cells may have formed in environments rich in organic compounds, such as primordial soups or deep-sea hydrothermal vents.

The cells absorbed nutrients from their surroundings, gradually evolving mechanisms to harness energy from available organic molecules. The key point here is that these early cells did not produce their own organic molecules but relied on external sources.

2. **Autotrophic Origin**: In contrast, the autotrophic origin hypothesis proposes that the first cells were autotrophic, meaning they could synthesize their own organic molecules from inorganic sources, such as carbon dioxide.

These early cells might have utilized energy from sources like sunlight or chemical reactions to drive the synthesis of complex organic compounds. Unlike heterotrophic cells, autotrophic cells could produce their own energy and carbon-containing molecules independently of external sources.

The main difference between these hypotheses lies in how they suggest early cells obtained organic molecules: heterotrophic cells absorbed them from the environment, while autotrophic cells synthesized them internally.

the following is an example of an indirect observation?
A.
using tools such as a thermometer or rain gauge to take measurements
B.
counting rings on a tree stump to determine its age
C.
counting the number of bald eagles that land near a river
D.
using a live video to observe nesting owls

Answers

D. Using a live video to observe nesting owls

Which of the following reasons best explains why a scientist would want to examine a possible link between modern women waiting longer to have children and an increase in the number of female babies?
A.
to test the predictions of current theories
B.
to discover new aspects of the natural world
C.
to test the conclusions of prior investigations
D.
to explain recently observed phenomena
Reset Submit

Answers

Answer:

D

Explanation:

Brain

A scientist performs scientific investigations to test the hypothesis and reach a conclusion. The best reason for examining the link includes an explanation of the recently observed phenomena. Thus, option D is correct.

What are scientific investigations?

Scientific Investigation is defined as the discovery of new information that involves asking questions and researching to solve the problem and reach a solution. It involves testing the hypothesis that begins with observations.

The observation that women tend to give birth at late ages and the increase in the number of the frame population are used to conduct the scientific investigation so as to prove and explain the observed phenomena.

Therefore, option D. the scientific investigation uses the observed phenomena to explain the information.

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economic importance of scorpion​

Answers

Explanation:

ANTIVENOM PRODUCTION: venomous scorpion species are used to produce antivenom for treating enevenomation, safeguarding human health in regions where these arachnids are a threat. TOURISM: scorpion-centric eco-tourism can stimulate local economies through guided tours and related services

what type of commensal relationship is orchids growing on branches of trees ?​

Answers

The relationship between orchids and the tree would be commensalism. Orchids receive nutrients from the air and compost on the tree. The tree, however, is neither harmed nor helped by the orchids.

How does energy get transferred from the Sun to a cow?

Answers

Answer:the sun provides food for he cow because it makes food grow

Explanation:

What was first responsible for changing the formerly poisonous atmosphere to an oxygen-rich atmosphere, as we have today?

Answers

Answer:

"Photosynethic bacteria (Cyanobacteria)" was first responsible for changing the formerly poisonous atmosphere to an oxygen-rich atmosphere, as we have today

Explanation:

The cyanobacteria are photosynthetic bacteria that are oxygenic. They extract energy from the sun, take up carbon dioxide and release oxygen. Unlike plants and algae, cyanobacteria comprise chlorophyll, and by carbon fixation transform carbon dioxide into sugar.

Cyanobacteria are prokaryotic species, unlike eukaryotic plants and algae. A phylum composed of free-living bacteria and endosymbiotic plastids (a Gloeomargarita sister group found in certain eukaryotes) is called as Cyanophyta or Cyanobacteria. These also contain multiple yellowish carotenoids, the blue phycobilin pigment and, in certain organisms, the red phycoerythrin pigment.

What are the roles of producers consumer, and decomposers in an ecosystem

Answers

Answer:

Producers Create Energy for the Consumers

Consumers "Use" or Consume Things of the environment

Decomposes eat or destroy the dead consumers and producers to help the environment grow

Answer:

thank youu!

Explanation:

What is malaria ?❤️‍♀️

Answers

fever caused by a protozoan parasite that invades the red blood cells.

Answer:

a tropical disease caused by a parasite

Explanation: it it a mosiqto bite from tropical islands

Which of the following Gametes can be formed from the following genotype: TtYy.

A. YY
B. tY
C. ty
D. TY
E. Ty
F. Tt

Answers

Gametes can be formed from the following genotype: TtYy are

B. tY

C. ty

D. TY

E. Ty

Explanation:

The gametes are formed by meiosis of the cell.

The cell with genotype TtYy will form four different types of gametes.

The cross is shown in the diagram attached.

The alleles of TtYy separates and form gametes by all possible combinations.

Four types of gametes formed by TtYy are - tY, ty, TY, Ty

What covers the ends of bones in adults?
A blood
B cartilage
C marrow
D ligaments

Answers

Answer is

Cartilage

Answer: cartilage

Explanation: Cartilage is a type of connective tissue, tough and flexible that serves as a protection to the ends of the bones and allows them to move. Cartilage is made up of a dense network of collagen fibres in a gelatinous substances. There are three main types of cartilage: hyaline cartilage, elastic cartilage and fibrocartilage.

The images show two species of tree frogs in a particular region.


The gray tree frogs are adapted to the trees of the woodlands, while the green tree frogs can survive in woodlands or grasslands. The gray frog was abundant in the woodlands. Because of climatic changes, most of the trees in the region died, and the woodlands changed to grasslands. In such a case, the .

The gray tree frog is closely related to the green tree frog genetically, but they have different mating calls. This suggests that specia

Answers

Answer:

Is adapting a new climate change

Explanation:

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Answer:

The gray tree frogs are adapted to the trees of the woodlands, while the green tree frogs can survive in woodlands or grasslands. The gray frog was abundant in the woodlands. Because of climatic changes, most of the trees in the region died, and the woodlands changed to grasslands. In such a case, the population of gray tree frogs will likely decrease .The gray tree frog is closely related to the green tree frog genetically, but they have different mating calls. This suggests that speciation occurred because of prezygotic isolation

Explanation:

The terrain is changing to a point in which it would be unsuitable for grey tree frogs and highly suitable for green tree frogs.

Prezygotic isolation prevents the fertilization of eggs. If the two frogs have different mating calls then they won't mate with eachother.

Which is a component of a phospholipid?
carbon ring
• hydrophilic head
long-chain alcohol
amino acid

Answers

The hydrophobic head is a component of a phospholipid

Answer:

b

Explanation:

Which of the following types of solutions should be used when preparing a wet mount? (Circle one.) a. hypertonic b.hypotonic c.isotonic

Answers

The solution of wet mount should be hypertonic solution.

Explanation:

Wet mount is a preparation where the specimen is made to place in a glass slide and a drop of solution is put on the slide. The wet mount is primarily used to see vaginal swab, bacterial slides etc.

Here the wet mount uses a hypertonic solution which is primarily 30 to 40% salt solution in distilled water. The function of this solution is to draw water from the cells and to keep them dehydrated.

The dehydration of the cells maintain their shape, and help to keep any stain if provided. So wet mount uses a hypertonic solution.

The moon rotates around the earth at a speed of 2,288 miles per hour. Is the moon accelerating?

Answers

Answer:

Yes, the moon is accelerating.

Explanation:

The moon is known to orbit around the sun at an average speed of 2,288 miles an hour. This translates to about 3,386 kilometers an hour. Further, it means that through its whole period of acceleration, it travels a distance of about 1,423,000 miles or 2,290,000 kilometers.

How is dna molecule replicated

Answers

DNA replication is the process by which DNA makes a copy of itself during cell division. The first step in DNA replication is to 'unzip' the double helix structure of the DNA? molecule. ... The two separated strands will act as templates for making the new strands of DNA.

Answer:

"During DNA replication, the DNA molecule separates into two strands, and then produces two new complementary strands following the rules of base pairing. Each case strand of the double helix of DNA serves as a template, or model, for the new strand."

Explanation:

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